Issue release date: 12.11.2024
Turkish Neurosurgery
2021 , Vol 31 , Num 2
Cytotoxic Effect of Boron Application on Glioblastoma Cells
1Kutahya University of Health Sciences, Department of Neurosurgery, Kutahya, Turkey2Kutahya University of Health Sciences, Department of Medical Services and Techniques, Kutahya, Turkey
3Dokuz Eylul University, School of Medicine, Department of Neurosurgery, Izmir, Turkey
4Yunus Emre State Hospital, Department of Neurosurgery, Eskisehir, Turkey
5Eskisehir Osmangazi University, School of Medicine, Department of Neurosurgery, Eskisehir, Turkey DOI : 10.5137/1019-5149.JTN.30316-20.1 AIM: To investigate the cytotoxic effects of boron application at different doses on U-87 MG glioblastoma cells.
MATERIAL and METHODS: The T98G (ATCC® CRL-1690?) glioblastoma cell strain used in the study was acquired from the American Type Culture Collection (ATCC) (Manassas, USA). Boric acid solution was prepared by mechanical mixing in the medium. Afterwards, 2.5 mM, 25 mM and 50 mM boron were each added to U87-MG glioblastoma cells and incubated for 48 hours. The cytotoxic effects on the cells was determined using the MTT (Methylthiazole diphenyl tetrazolium) test 48 hours after boron application.
RESULTS: IC50 value was detected as 17 mM in the 48-hour boric acid application on U-87 MG glioblastoma cells.
CONCLUSION: Boron treatment might be an effective approach for glioblastoma.
Keywords : Boron treatment, Glioblastoma, Cytotoxic effects