Turkish Neurosurgery 2011 , Vol 21 , Num 4
The Effect of Nitrogen Mustard on the Enzymatic Antioxidant Defense of Rat Brain Tissue and the Therapeutic Value of Proanthocyanidin
Ayhan TEKINER1, Dogan YUCEL2, Mehmet Akif BAYAR1, Orhan YUCEL3, Yavuz ERDEM1, Jale KARAKAYA4
1Ministry of Health, Training and Research Hospital , Department of Neurosurgery, Ankara, Turkey
2Ministry of Health, Training and Research Hospital, Medical Biochemistry, Ankara, Turkey
3Glhane Millitary Medical Academy, Department of Thoracic Surgery, Ankara, Turkey
4Hacettepe University, Faculty of Medicine, Department of Biostatistics, Ankara, Turkey
DOI : 10.5137/1019-5149.JTN .4028-10.3 AIM: Nitrogen Mustard (NM) is an alkylating agent that damages cellular nuclear DNA after penetrating tissue. This results in cytostatic, mutagenic and cytotoxic effects. We used biochemical analyses to investigate the effect of NM gas administered through the dermal and respiratory routes, on the brain cortex of rats and also tried to show whether the antioxidant Proanthocyanidin (PC) could decrease this effect.

MATERIAL and METHODS: A total of 30 rats were randomized into three groups: Group I: Control group, Group II: NM group, and Group III: NM + PC group. The rats were sacrificed 3 days after NM gas exposure. A segment of the cortical tissue was prepared for biochemical analyses.

RESULTS: Biochemical analyses of cortical neural tissue regarding the Enzymatic Antioxidant Defense against NM were performed. The results of these analyses implied that PC was effective for healing of cortical neural tissue.

CONCLUSION: These findings imply that structural changes induced by mustard gas can be prevented and restored by proanthocyanidin treatment. Keywords : Nitrogen mustard, Antioxidants, Proanthocyanidin, Rat brain

Corresponding author : Ayhan Tekner, tekinerayhan@hotmail.com