Turkish Neurosurgery 2011 , Vol 21 , Num 4
Hippocampal Neuronal Damage in Rats Exposed to a Double Hit: Irradiation and Hyperthermia
Gozde ERKANLI SENTURK1,2, Cihan ISLER3, Rahsan KEMERDERE4, Mustafa Onur ULU5, Cenk UMAY6, Aydan OZKAYMAKCI7, Gulhan Rezzan AKER7, Feriha ERCAN1, Cigdem OZKARA8, Filiz ONAT7, Mustafa UZAN4
1Marmara University, Faculty of Medicine, Department of Histoogy and Embryology, Istanbul, Turkey
2Acibadem University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey
3Bagcilar Education and Training Hospital, Department of Neurosurgery, Istanbul, Turkey
4Istanbul University, Cerrahpasa Faculty of Medicine, Department of Neurosurgery, Istanbul, Turkey
5Sakarya ToyotaSA State Hospital, Department of Neurosurgery, Istanbul, Turkey
6Istanbul University, Cerrahpasa Faculty of Medicine, Department of Radiation Oncology, Istanbul, Turkey
7Marmara University, Faculty of Medicine, Department of Pharmacology and Clinical Pharmacology, Istanbul, Turkey
8Istanbul University, Cerrahpasa Faculty of Medicine, Department of Neurology, Istanbul, Turkey
DOI : 10.5137/1019-5149.JTN .4012-10.1 AIM: In utero irradiation models induce diffuse neuronal damage. Experimental studies have shown that hyperthermia induced seizures are easily elicited and have high mortality accompanied by neuronal loss. Neuronal damage and loss are the results of cell death coupled with cortical development in altered cellular development. The aim of the study was to investigate the changes in hippocampus that was exposed to irradiation and hyperthermia.

MATERIAL and METHODS: Four groups were studied: 1) The irradiation group was exposed to 225 cGy irradiation on the 17th gestational day; 2) The hyperthermia group was exposed to hyperthermia on the 10th postnatal day; 3) The hyperthermia plus irradiation group was exposed to in utero irradiation and postnatal hyperthermia; 4) The control group was sham operated. Animals were examined 3 and 6 months later.

RESULTS: The hippocampus was atrophic with neuronal loss in CA regions and ectopic neurons were in irradiation group. Severe damage with the most atrophy was demonstrated in all regions of the irradiation plus hyperthermia group. In long term, damage was severe in all groups.

CONCLUSION: This study demonstrated more damage in hippocampi exposed to both irradiation and hyperthermia that may be taken as an evidence for the double hit hypothesis in the development of hippocampal damage. Keywords : Hippocampus, Hyperthermia, Irradiation, Neuronal damage, Rat

Corresponding author : Gozde Erkanlř Senturk, gozdeerkanli@yahoo.com