Turkish Neurosurgery 2009 , Vol 19 , Num 4
The Effect of Nitrogen Mustard on the Rat Brain and the Therapeutic Value of Proanthocyanidin
Ayhan TEKINER1, Orhan YUCEL2, Ayse KOSE SARGIN3, Onur GENC4, Belgin CAN5, Turhan KARAYILANOGLU6, Jale KARAKAYA7, Mehmet Akif BAYAR8
1,8Ministry of Health, Training and Research Hospital, Department of Neurosurgery, Ankara, Turkey
2,4Glhane Military Medical Academy, Department of Thoracic Surgery, Ankara, Turkey
3,5Ankara University School of Medicine, Department of Histology and Embryology, Ankara, Turkey
6Glhane Military Medical Academy, Department of Nuclear, Biologic and Chemical Warfare, Ankara, Turkey
7Hacettepe University, School of Medicine, Department of Biostatistics, Ankara, Turkey
AIM: Nitrogen Mustard (NM) is an alkylating agent that damages cellular nuclear DNA after penetrating tissue. This results in cytostatic, mutagenic and cytotoxic effects. We used the electron microscope to investigate the effect of NM gas administered through the dermal and respiratory routes to rats on the brain cortex and also tried to show whether the antioxidant Proanthocyanidin (PC) could decrease this effect.

MATERIAL and METHODS: Atotal of 32 rats were randomized into four groups: Group I: Control group, Group II: PC group, Group III: NM group, Group IV: NM + PC group. The rats were sacrificed 3 days after NM gas exposure. A segment of the cortical tissue was prepared for electron microscopy.

RESULTS: We used the electron microscope for cellular analysis of NM on cortical neural cells. These investigations revealed degeneration of the cortical neural cell nuclei together with oedema and axonal degeneration in the subcortical neural tissue. The group receiving antioxidants was found to have less oedema and degeneration.

CONCLUSION: These findings imply that structural changes induced by mustard gas can be prevented and restored by proanthocyanidin treatment. Keywords : Nitrogen Mustard, Proanthocyanidin, Rat brain

Corresponding author : Ayhan Tekner, tekinerayhan@hotmail.com