Turkish Neurosurgery 2016 , Vol 26 , Num 4
The Neuroprotective Effects of Resveratrol Preconditioning in Transient Global Cerebral Ischemia–Reperfusion in Mice
Shuang LIU, Jinbo SUN, Yanshuang LI
Jinan Central Hospital Affiliated to Shandong University, Department of Neurology, Jinan, China DOI : 10.5137/1019-5149.JTN.14195-15.3 AIM: This study was designed to elucidate the neuroprotective effect of resveratrol in a mouse model of bilateral common carotid artery occlusion (BCCAO).

MATERIAL and METHODS: Sixty male C57BL/6 mice, weighing 20–24 g, were used in our experiments. The mice were randomly assigned into three groups: control group, BCCAO group and BCCAO+Resveratrol group. Neurological score was assessed 24h, 48h, 72h after BCCAO, respectively. Hematoxylin and eosin (H&E) staining, NeuN and TUNEL were performed to detect the neuronal death and survival. The expression of Bcl-2, Bax, caspase-3, and cleaved caspase-3 were also detected to assess the anti-apoptotic effect of resveratrol by Western Blot.

RESULTS: Resveratrol significantly improved neurological score in BCCAO mice. Besides, it attenuates neuronal apoptosis via increasing the expression of Bcl-2 and decreasing the expression of Bax, caspase-3, and cleaved caspase-3. Resveratrol promotes neuronal survival in mice subjected to BCCAO.

CONCLUSION: Resveratrol is beneficial in the model of BCCAO, which is associated with its anti-apoptotic effect. Keywords : Resveratrol, Apoptosis, Global cerebral ischemia

Corresponding author : Shuang Lu, liushuang201577@163.com