Turkish Neurosurgery 2013 , Vol 23 , Num 4
Investigation of ACE Genome Insertion/Deletion Correlation with Immunohistochemical Profile in Pituitary Adenomas
Murat ALTAS1, Omer Faruk BAYRAK2, Alpay Seref HAN3, Gokalp SILAV4, Kerim Kenan COSKUN3, Ajlan CERCI3, Nejat ISIK3, Ţlhan ELMACI4
1Mustafa Kemal University, Faculty of Medicine, Department of Neurosurgery, Antakya, Turkey
2Yeditepe University, Faculty of Medicine, Department of Genetics and BioEngineering, Istanbul, Turkey
3Goztepe Education and Research Hospital, Department of Neurosurgey, Istanbul, Turkey
4Acibadem University, Faculty of Medicine, Department of Neurosurgey, Istanbul, Turkey
DOI : 10.5137/1019-5149.JTN.7423-12.0 Aim: The deletion polymorphism of the angiotensin-converting enzyme (ACE) genome causes neoplastic development in several organs by increasing the angiotensin 2 (A2) formation. In this study, we aimed to identify the ACE genome insertion/deletion polymorphism in pituitary adenomas and to compare it with the control group.

Material and Methods: Patients operated for pituitary adenomas were included in the study. Genomic DNA was extracted from tumoral tissues and peripheral blood samples of the patients by using the Miller method. Primary sequence was selected via targeting the polymorphic region of intron 16 of ACE genome 17q23. DNA samples were multiplied by PCR using HACE3s and HACE3as primers.

Results: Twenty-one operated cases were studied. In the study group; 44 % of the patients were identified as D/D, 33% of them as I/D and 23% of them as I/I. In 60%, D allele was identified. According to immunohistochemical investigation, we found that 100% of the patients with Cushing adenoma were D/D alleles.

Conclusion: Presence of high rate of ACE genome deletion in patients with pituitary adenoma and grade 3-4 patients suggest that ACE genome polymorphism can be a risk factor for the development of pituitary adenomas. Keywords : Angiotensin-converting enzyme, Pituitary adenomas, Gene

Corresponding author : Murat Altas, draltasmurat@yahoo.com