Turkish Neurosurgery 2023 , Vol 33 , Num 6
The Synergistic Effect of Theranekron and Cisplatin on the Neuroblastoma (SH-SY5Y) Cell Line
1Usak University, Faculty of Medicine, Department of Medical Pharmacology, Usak, Türkiye DOI : 10.5137/1019-5149.JTN.43431-23.4 AIM: To assess the combined antitumor potential of theranekron (Trn) and cisplatin in neuroblastoma (NB), a pediatric tumor characterized by high prevalence and mortality rates. The investigation involved examining the interaction between theranekron (10-100 ?M) and cisplatin (40 ?M) within this cell line.

MATERIAL and METHODS: The cytotoxicity of Trn was evaluated using MTT assays, cell volume analyses, apoptosis assessments, and measurements of mitochondrial membrane potential. These evaluations were conducted over a 24-hour incubation period.

RESULTS: The coadministration of 100 µM Trn with cisplatin exhibited a cytotoxic effect of approximately 60%. Furthermore, it led to a reduction of up to 38% in cell volume. Notably, SH-SY5Y cells demonstrated an early apoptosis rate of 34.4%, accompanied by an eightfold decrease in mitochondrial membrane potential compared to the control group.

CONCLUSION: Trn demonstrated synergism and significantly enhanced the efficacy of cisplatin (p<0.001). Nevertheless, further in-depth investigations are necessary to elucidate the intricacies of these synergistic effects. Keywords : Cisplatin, SH-SY5Y, Synergism, Theranekron

Corresponding author : Numan TASPINAR, numan.taspinar@usak.edu.tr