Turkish Neurosurgery 2023 , Vol 33 , Num 6
Knockdown of the Long Noncoding RNA CRNDE Ameliorates Apoptosis and Inflammation in Ischemia-Reperfusion-Induced Brain Injury via the mir-489-3p/FOXO3 Pathway
Yinbao HU1,Min LI1
1Yantai Yuhuangding Hospital Affiliated to Qingdao University, Department of Neurology, Yantai 264000, Shandong, China DOI : 10.5137/1019-5149.JTN.36652-21.2 AIM: To examine the role and mechanism of colorectal tumor differential expression (CRNDE) in brain injury induced by ischemicreperfusion.

MATERIAL and METHODS: Sh-SY5Y cells were cultured, and oxygen and glucose deprivation/reperfusion (OGD/R) injury tests were performed. The effects on SH-SY5Y cells were evaluated by the Cell Counting Kit-8 (CCK-8) assay, qPCR, apoptosis analysis, western blot analysis, ELISA, a luciferase reporter assay, and an RNA pull-down assay.

RESULTS: Knockdown of CRBDE ameliorated SH-SY5Y cell impairment induced by OGD/R. CRNDE, the target of mir-489-3p, was directly bound to FOXO3. Mir-489-3p knockdown partially reversed OGD/R-mediated impairment in CRBDE knockdown SH-SY5Y cells.

CONCLUSION: The results indicate that knockdown of lncRNA CRNDE ameliorates apoptosis and the inflammatory response in ischemia-reperfusion-induced brain injury through the mir-489-3p/FOXO3 axis. LncRNA CRNDE may represent a novel therapeutic target for brain injury. Keywords : CRNDE, Ischemia-reperfusion, Apoptosis, mir-489-3p, FOXO3

Corresponding author : Min LI, retk225@163.com