Turkish Neurosurgery 2022 , Vol 32 , Num 6
Expression and Role of CXCL13 and MiR-186-5p in the Trigeminal Ganglion of a Rat Model of Trigeminal Neuralgia
Linfei FENG1,Liangxin YANG2,Xiufang SHI2,Liecheng WANG3,Yuanyin WANG2
1The First Affiliated Hospital of Anhui Medical University, Department of Oral and Maxillofacial Surgery, Hefei, China
2Anhui Medical University, College of Stomatology, Hefei, China
3Anhui Medical University, School of Basic Medical Sciences, Hefei, China
DOI : 10.5137/1019-5149.JTN.36642-21.2 AIM: To reveal the roles of CXCL13 and miR-186-5p in a rat model (adult male Sprague-Dawley rats, 7?8 weeks old, 180?200 g) of trigeminal neuralgia (TN) established via chronic constriction injury of the infraorbital nerve (ION-CCI).

MATERIAL and METHODS: The results of behavioural tests and the expression levels of miR-186-5p and CXCL13 in the trigeminal ganglion (TG) were compared between the sham and ION-CCI groups, as well as the consequences of the miR-186-5p mimic and inhibitor.

RESULTS: Compared with the sham-operated rats, ION-CCI rats displayed mechanical hypersensitivity in the von Frey hair test. Western blotting revealed the upregulation of CXCL13 and downregulation of miR-186-5p in the TG of ION-CCI rats relative to their expression in sham rats. Furthermore, an miR-186-5p mimic decreased CXCL13 protein levels and increased the mechanical withdrawal thresholds of ION-CCI rats. CXCL13 protein levels also increased after the injection of an miR-186-5p inhibitor. Finally, miR-186-5p was found to be expressed in the TG and was downregulated in ION-CCI rats compared to sham rats.

CONCLUSION: miR-186-5p may negatively regulate CXCL13 to influence the occurrence and development of TN. Collectively, our findings shed new light on novel therapies for the treatment of TN. Keywords : MiR-186-5p, CXCL13, Trigeminal neuralgia, Trigeminal ganglion, Chronic constriction injury

Corresponding author : Liecheng WANG, wangliecheng@ahmu.edu.cn