Turkish Neurosurgery 2022 , Vol 32 , Num 6
The Effects of Necrostatin-1 on Cerebral Vasospasm- Induced Subarachnoid Hemorrhage
Mehmet Hakan SAHIN1,Mehmet Emin AKYUZ1,Hakan Hadi KADIOGLU1
1Ataturk University, School of Medicine, Department of Neurosurgery, Erzurum, Turkey DOI : 10.5137/1019-5149.JTN.35167-21.4 AIM: To investigate the effects of necrostatin-1 (NEC-1) on subarachnoid hemorrhage (SAH)-induced vasospasm in a rat model.

MATERIAL and METHODS: A total of 59 male Wistar albino rats were randomly divided into six groups: control (group 1), sham (group 2), decapitation one hour after SAH (group 3), decapitation 48 hours after SAH (group 4), NEC-1 given 15 minutes before SAH and decapitation one hour after SAH (group 5), and NEC-1 given 24 hours after SAH and decapitation 48 hours after SAH (group 6). NEC-1 (1 uq) was administered intracisternally in dimethyl sulfoxide (DMSO) (2.6 uq). After decapitation, the cross-sectional areas and wall thicknesses of basilar arteries were determined histopathologically using stereological methods.

RESULTS: NEC-1 administered before SAH had a statistically significant preventive effect on vasospasm following SAH. Arterial wall thicknesses were found to be significantly increased in the SAH without NEC-1 groups but not in the control group, the sham group or the NEC-1 groups.

CONCLUSION: The results of this study show that NEC-1 can prevent vasospasm in rats and has cytoprotective effects. Further studies are needed for the clinical use of this agent. Keywords : Necrostatin-1, Stereology, Subarachnoid hemorrhage, Vasospasm, Rat

Corresponding author : Mehmet Hakan SAHIN, mhsahin21@hotmail.com