Turkish Neurosurgery 2021 , Vol 31 , Num 1
Effect of Paracetamol in the Proliferation of Glioblastoma Cell Line: The Role of Apoptosis, COX-2 and Cyclin B Expressions
Ersoy OKSUZ1,Gokhan GORGISEN2,Hulya OZDEMIR1,Ismail Musab GULACAR2,Gokhan OTO1
1Van Yuzuncu Yıl University, School of Medicine, Department of Medical Pharmacology, Van, Turkey
2Van Yuzuncu Yıl University, School of Medicine, Department of Medical Biology, Van, Turkey
DOI : 10.5137/1019-5149.JTN.27866-19.7 AIM: To investigate the relationship between paracetamol and expression levels of cyclooxygenase-2, cyclin B, cell viability and apoptosis in glioblastoma cell line.

MATERIAL and METHODS: The A172 glioblastoma cells were treated with different concentrations of paracetamol and phosphate buffer saline as a vehicle for 24, 48, and 72 hours. Cell viability was detected by MTT. Bax, procaspase 3, COX-2 and Cyclin B expressions were detected using Western blotting.

RESULTS: A paracetamol treatment of 0.5 mg/mL for 24, 48, and 72 hours led to a 14%, 31%, and 37% decrease in cell viability. The expression of COX-2 and cyclin B levels decreased by 36% and 52% respectively, after treatment with 0.5 mg/mL paracetamol. Treatment with 0.5 mg/mL and 1 mg/mL paracetamol significantly induced the expression of cleaved caspase 3, procaspase 3 and Bax proteins compared to the control group (60%, 40%, 21%, %100, 18%, 17%, respectively).

CONCLUSION: The results of our study showed that paracetamol has antitumoral effects on glioblastoma cells and this activity was induced by different signaling pathways. Keywords : Paracetamol, Glioblastoma, COX-2, Cyclin B, Apoptosis, A172 glioblastoma cells

Corresponding author : Ersoy OKSUZ, drugoksuz@hotmail.com