Turkish Neurosurgery 2019 , Vol 29 , Num 5
Immunohistochemical Evaluation of Hemostatic Changes in Glioblastoma Multiforme and Low-Grade Astrocytoma
1Heinrich-Braun-Clinic, Department of Neurosurgery, Zwickau, Germany
2Cukurova University, School of Medicine, Balcali Hospital, Department of Neurosurgery, Adana, Turkey
3Friedrich- Schiller University, Department of Neurosurgery, Jena, Germany
4Cukurova University, School of Medicine, Balcali Hospital, Department of Pathology, Adana, Turkey
5Acibadem Adana Hospital, Neurosurgery Clinic, Adana, Turkey
DOI : 10.5137/1019-5149.JTN.22739-18.3 AIM: To compare glioblastoma multiforme with astrocytoma grade II by subjectively evaluating the levels of prothrombin and biotinylation thrombin, and G protein serum protease activatin receptors, as tissue factors causing hypercoagulation and affecting coagulation.

MATERIAL and METHODS: Specimens from 35 cases with glioblastoma multiforme and 23 cases with astrocytoma grade II were evaluated immunohistochemically. The specimens were stained with hematoxylen-eosin and immunohistochemically for prothrombin, biotinylation thrombin and protease activating factor receptors to determine the correlation between the tumor malignancy and coagulation factor receptors.

RESULTS: An increase in malignancy was seen to result in an increase in prothrombin, biotinylation thrombin, protein activator receptor 1, protein activator receptor 3, and protein activator receptor 4 levels, and a decrease in protein activator receptor 2 level. These data showed that there was hypercoagulability in glioblastoma multiforme. Descriptive statistics and Mann-Whitney U analysis were used to evaluate the results.

CONCLUSION: In glioblastoma multiforme with no radiological evidence of hemorrhage, low molecular weight heparin should be administered peroperatively and continued for 3 months postoperatively to prevent the development of deep venous thrombosis. This will also be useful in the prevention of invasion, angiogenesis, metastasis and tumour progression. Keywords : Gliblastoma multiforme, Astrocytoma, Prothrombin, Thrombin, Protein activator receptor, Hypercoagulability

Corresponding author : Dervis Mansuri YILMAZ, mansuriyilmaz@gmail.com