Turkish Neurosurgery 2016 , Vol 26 , Num 6
Intrathecal Administration of Flavopiridol Promotes Regeneration in Experimental Model of Spinal Cord Injury
Chang LI1, Jian ZHANG2, Pei-Qian ZHU3, Chang-Hua MA1, Lin-Hui YUAN1, Jun LU1, Zhen-Zhong LUO1, Guo-Hai XU1
1Second Affiliated Hospital of Nanchang University, Department of Anesthesiology, Nanchang, China
2Nanjing Medical University, Huai’an First People’s Hospital, Department of General Surgery, Huai’an, China
3Second Affiliated Hospital of Nanchang University, Department of General Surgery, Nanchang, China
DOI : 10.5137/1019-5149.JTN.13745-15.2 AIm: Spinal cord injury (SCI) is a serious condition of the central nervous system and it affects the quality of life and even hampers the day-to-day activity of the patient. In the current study, we investigated the efficacy of intrathecal administration of flavopiridol in an experimental animal model of SCI. The study also aimed at exploring the physiological effects of flavopiridol on neurons, astrocytes and cell cycle regulatory proteins.

MaterIal and Methods: In vitro scratch wound experiments were performed on female Sprague–Dawley rats (n=23). A complete hemisection to the right of T10 was made, and flavopiridol solution (200 mM, 0.8 nmol flavopiridol/animal) was delivered topically to the lesion site. Cell viability assay, in vitro scratch injury assay, cell cycle analysis using flow cytometry and behavioural assessments were performed.

Results: The local delivery of flavopiridol reduced cavity formation and improved regeneration of neurons with improvement in physiological performance. Flavopiridol also inhibited the migration and proliferation of astrocytes, and at the same time, promoted the survival of neurons.

ConclusIon: Intrathecal administration of flavopiridol can be a promising treatment strategy in patients with SCI and it needs to be validated in patient setting. Keywords : Spinal cord injury, Intrathecal injections, Flavopiridol

Corresponding author : Guo-hai Xu, xuguhai791@gmail.com