Turkish Neurosurgery 2016 , Vol 26 , Num 2
Evaluation of Computed Tomography Perfusion Imaging in a Rat Acute Cerebral Ischemia-Reperfusion Model
Xiuhua MA1, Hua LI2, Zhibo XIAO3, Mingquan LI4, Peng XUE1, Yong CHEN1, Jigang ZHONG1
1Zhengzhou People’s Hospital, Department of Radiology, Zhengzhou, China
2Rizhao City People’s Hospital, Department of Radiology, Rizhao, China
3Chongqing University of Medical Sciences, The First Affiliated Hospital, Department of Radiology, Chongqing, China
4Luohe City People’s Hospital, Department of Radiology, Luohe, China
DOI : 10.5137/1019-5149.JTN.12938-14.2 AIM: This study aims to apply computed tomography perfusion imaging (CTPI) technology under in vivo conditions in order to explore its reliability and accuracy in evaluating the rat acute cerebral ischemia-reperfusion model (RACIRM).

MATERIAL and METHODS: The thread embolism method was used in 48 rats to create the RACIRM. Rats were divided into 2 groups as ischemia group and ischemia-reperfusion group. We then compared and evaluated the results of CTPI, 2,3,5-triphenyl tetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining of both groups.

RESULTS: There were no significant differences in the volumes of hypoperfusion regions in the cerebral blood flow (CBF) and cerebral blood volume (CBV) of each group at each CTPI time point and these volumes were not significantly different from the corresponding findings on the TTC-stained infarct regions. The mean transit time (MTT) did show a significant difference, as did the volumes observed in both the MTT ischemic region and TTC-stained infarct region. The CTPI parameters exhibited correlation with the infarct volumes calculated in TTC staining, among which CBV exhibited the highest correlation.

CONCLUSION: CTPI could rapidly, accurately, and non-invasively evaluate the site, size, and hemodynamic changes in the cerebral ischemia-reperfusion animal model. Keywords : Computed tomography perfusion imaging, Acute cerebral ischemia, Cerebral hemodynamics, Rat

Corresponding author : Xiuhua Ma, xiuhuamacn@163.com