Turkish Neurosurgery 2024 , Vol 34 , Num 6
Trolox Reduces Neuroblastoma-Induced Oxidative Stress and Inflammation
Haydar CELIK1,Guven AKCAY2,Aysenur BUDAK SAVAS3,Fatma YESILYURT3,Filiz DEMIRDOGEN4,Dilanur ATES3,Ahmet HACIMUFTUOGLU3
1Ankara Research and Training Hospital, Department of Neurosurgery, Ankara, Türkiye
2Bolu Abant İzzet BaysalUniversity, Faculty of Medicine, Department of Biophysics, Bolu, Türkiye
3Atatürk University, Faculty of Medicine, Department of Medical Pharmacology, Erzurum, Türkiye
4Private Neon Hospital, Department of Neurology, Erzincan, Türkiye
DOI : 10.5137/1019-5149.JTN.46442-24.4 AIM: To determine the effects of different concentrations of Trolox on the cytotoxic, oxidant, antioxidant, and cytokine levels in the SH-SY5Y cell line.

MATERIAL and METHODS: SH-SY5Y cells were grown in an appropriate medium and under appropriate conditions. Trolox was added to the cell line at concentrations of 50, 100, 200, 400 and 800 ?M and incubated for 24 and 48 hours. Subsequently, methyl thiazolyl tetrazolium (MTT) and proinflammatory cytokine level assays were performed, and the cytotoxicity of oxidative stress was assessed.

RESULTS: Administration of 200 ?M of Trolox reduced the cancer cell viability to 77.76%. Furthermore, Trolox exhibited a concentration-dependent effect on the SH-SY5Y cell line. Administration of 200 ?M of Trolox also reduced the oxidant activity, increased the antioxidant capacity, and decreased the proinflammatory cytokine levels in neuroblastoma (NB) cells, which were consistent with the cytotoxicity test results.

CONCLUSION: The results of this experimental study demonstrated a Trolox concentration of 200 ?M produces an anticancer effect on NB cell-line. Keywords : Inflammatory Neuroblastoma, Oxidative stress, Trolox

Corresponding author : Haydar CELIK, drhaydarcelik1976@gmail.com