Turkish Neurosurgery 2024 , Vol 34 , Num 3
The Effect of Slime Factor in the Treatment of Spinal Implant Infections
Hasan TURKOGLU1,Emrullah Cem KESILMEZ2,Kutsal Devrim SECINTI2,Ilke Evrim SECINTI3,Burak KUCUK4,Murat ARAL4, Recep EKEN5, Zafer YUKSEL2
1Ersin Arslan City Hospital, Department of Neurosurgery, Gaziantep, Türkiye
2Kahramanmaras Sutcu Imam University, Faculty of Medicine, Department of Neurosurgery, Kahramanmaras, Türkiye
3Hatay Mustafa Kemal University, Faculty of Medicine, Department of Pathology, Hatay, Türkiye
4Kahramanmaras Sutcu Imam University, Faculty of Medicine, Department of Microbiology, Kahramanmaras, Türkiye
5Adiyaman Research Hospital, Department of Neurosurgery, Adiyaman, Türkiye
DOI : 10.5137/1019-5149.JTN.43753-23.1 AIM: To investigate the effect of the biofilm-forming ability of the bacteria on treatment in rats by using biofilm-forming and nonbiofilm- forming strains of Staphylococcus aureus (S. aureus).

MATERIAL and METHODS: Forty rats were divided into four equal groups as Group 1A, 1B, 2A, and 2B. All rats underwent single distance lumbar laminectomy, and titanium implants were introduced. Group 1 rats were inoculated with Slime factor (?) S. aureus, while Group 2 rats were inoculated with biofilm Slime factor (+) S. aureus. None of the rats were given antibiotics. One week later, the surgical field was reopened and microbiological samples were taken. The implants of rats in Groups 1A and 2A were left in place, while the implants of rats in Groups 1B and 2B were removed.

RESULTS: There was no statistically significant difference between the groups inoculated with slime factor (+) S. aureus; although, Groups 1A and 2A showed statistically significant difference. Statistical analysis with respect to bacterial count also showed a statistically significant difference between Groups 1A and 2A. There was a statistically significant difference between Group 1B and 2B.

CONCLUSION: The results obtained in the present study reveal that in case of implant-dependent infection, the first sample taken can be checked for slime factor, and if there is infection with slime factor-negative bacterium, treatment without removing the implant may be recommended. S. aureus was used in the study because it is the most common cause of implant-related infection at surgical sites. Further studies using different bacterial species are needed to reach a definitive conclusion. Keywords : Biofilm layer, Slime factor, Implant, Rat

Corresponding author : Emrullah Cem KESILMEZ, cemkesilmez@gmail.com