Turkish Neurosurgery 2024 , Vol 34 , Num 2
Effects of Focal Cerebellar Injury on Fracture Healing and Oxidative Stress in Rat Model: An Experimental Animal Study
Fatih DOGAR1,Kaan GURBUZ2,Duran TOPAK1,Aysun OKCESIZ3,Ayse EKEN3,Emine KILINC4,Mustafa ARIK5,Okkes BILAL1,Mustafa Abdullah OZDEMIR1,Mikail TELEK1
1Kahramanmaras Sutcu Imam University, Faculty of Medicine, Department of Orthopaedics and Traumatology, Kahramanmaras, Türkiye
2Kayseri City Education & Research Hospital, Clinical of Orthopedics and Traumatology, Kayseri, Türkiye
3Erciyes University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Kayseri, Türkiye
4Kahramanmaras Sutcu Imam University, Faculty of Medicine, Department of Pathology, Kahramanmaras, Türkiye
5Aksaray Ortakoy State Hospital, Clinical of Orthopedics and Traumatology, Aksaray, Türkiye
DOI : 10.5137/1019-5149.JTN.44200-23.2 AIM: To examine the effect of cerebellar damage on the process of fracture healing.

MATERIAL and METHODS: A total of forty-two male rats were selected at random and subsequently allocated into three distinct groups. The experimentals were divided into two subgroups within each group, with the intention of sacrificing them during the third and sixth weeks. Group 1 had isolated femoral fracture, Group 2 had femoral fracture after craniotomy, and Group 3 had femoral fracture accompanying cerebellar injury after craniotomy. Left femoral fractures in rats in all groups were treated using an intramedullary Kirschner wire. Radiological, histological, and biochemical evaluations were conducted at 3 and 6 weeks to assess the processes of fracture healing. To determine the effects of fracture healing and cerebellar injury on oxidant?antioxidant systems, catalase (CAT), malondialdehyde, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities were measured.

RESULTS: Between the time frame of 3 to 6 weeks, Group 3 had higher radiography scores, alkaline phosphatase levels, callus/ diaphyse ratio, callus improvement, and bone mineral density in comparison to the other groups. The activity of SOD was found to be statistically negligible in all groups, suggesting that SOD does not have a substantial impact on fracture healing in cerebellar injury. However, notable increases in the activity of GPx and CAT enzymes were observed, showing their considerable involvement in the process of fracture healing.

CONCLUSION: Cerebellar injury reduces the oxidative stress in the fracture area and contributes positively to fracture healing by means of radiologically, biochemically and histopathologically. Keywords : Cerebellar injury, Fracture healing, Antioxidant, Bone, Rats

Corresponding author : Fatih DOGAR, drfatihdogar@hotmail.com