Issue release date: 12.11.2024
2Sivas Cumhuriyet University, Faculty of Pharmacy, Department of Biochemistry, Sivas, Turkey DOI : 10.5137/1019-5149.JTN.30290-20.2 AIM: To investigate the neurotoxic effects of strontium (Sr) compounds and Glycyrrhiza glabra (licorice, G. glabra).
MATERIAL and METHODS: In this study, we conducted neurotoxicity assays on the human cortical neuronal cell line HCN-2 (CRL- 10742) to determine the potential neurotoxic effects of Sr and G. glabra.
RESULTS: No significant decrease in HCN-2 cell viability was observed with longer Sr exposure or Sr concentrations up to 2000 ?g/mL. The IC50 values of Sr for 24 and 48 hours of exposure were >2000 ?g/mL, and 936.9 ± 0.09 ?g/mL for 72 hours. However, we observed a significant reduction in HCN-2 cell viability with longer exposure and higher concentrations of G. glabra. The IC50 values of G. glabra for 24, 48, and 72 hours were 545.1 ± 0.03 ?g/mL, 398.1 ± 0.03 ?g/mL, and 393.3 ± 0.02 ?g/mL, respectively.
CONCLUSION: Additional studies are needed to further investigate the neurotoxicity of Sr and G. glabra, and elucidate the pathway by which these compounds exert their therapeutic effects in pathological conditions.
Keywords : Glycyrrhiza glabra, Neurons, Cell line, Human Brain Cortical Neuronal Cell line, Neurotoxicity, Strontium