Issue release date: 12.11.2024
2Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Neurosurgery, Istanbul, Turkey
3Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Medical Biology, Istanbul, Turkey DOI : 10.5137/1019-5149.JTN.29805-20.2 AIM: To investigate the effect of mitochondrial DNA (mtDNA) variants mainly in D-loop on glioma biology.
MATERIAL and METHODS: Sanger sequencing of D-loop (15971?16451 bp) for 52 glioma patients was performed and the variations were statistically analyzed for gender, WHO classification, morphological grade, IDH/TERT status.
RESULTS: Total of 122 variations (51 unique) were identified in 52 patients. C16223T, T16189C, T16311C and T16126C variants were frequently detected. The total variation number was statistically non-significant among the analyzed categories. When individual variants were considered, T16311C and T16224C were statistically significant for WHO classification (p=0.033), morphological grade (p=0.036) and gender (p=0.039), respectively.
CONCLUSION: Total variation number in D-loop was not found to be related with clinical variables. Our data suggests that individual variants may play a critical role in glioma biology.
Keywords : D-loop, Glioma, Mitochondrial DNA, mtDNA variations, Mutations