Issue release date: 12.11.2024
Turkish Neurosurgery
2021 , Vol 31 , Num 1
Practical Performance of Hippocampal Tissue Resection in Rats in Pharmacomolecular Research
1Istanbul Medipol University, School of Medicine, Department of Medical Pharmacology, Istanbul, Turkey2Namik Kemal University, School of Medicine, Department of Neurosurgery, Tekirdag, Turkey DOI : 10.5137/1019-5149.JTN.31730-20.1 AIM: To explain how to resect hippocampal tissue in rats used as live mammalian subjects for the resection of fresh cerebral tissue in laboratories.
MATERIAL and METHODS: Adult male Wistar-Albino rats (n=50) were used for this purpose.
RESULTS: The average bodyweight of the rats was 316.4 ± 11.71 g, and the average weight of the resected hippocampal tissues was 1.01 ± 0.03 g; however, there were no statistically significant differences between the body weights and the hippocampal tissue weights (p>0.05). The hippocampal tissues to be used in the study were excised practically by preserving the original anatomical configuration without injury to the tissue.
CONCLUSION: This paper elucidates a simple, step-by-step methodology for performance in the laboratory in order to improve the standardization of hippocampal tissue dissection.
Keywords : Dissection, Brain, Cerebellum, Cortex, Hippocampus, Wistar-Albino, Rat