Issue release date: 12.11.2024
2Ankara University, School of Medicine, Department of Biostatistics, Ankara, Turkey
3Ege University, School of Medicine, Department of Pediatrics, Izmir, Turkey DOI : 10.5137/1019-5149.JTN.26158-19.1 AIM: To investigate the effects of Phenyramidol (Phe) on neural development in an early chicken embryo model.
MATERIAL and METHODS: Sixty fertile non-pathogenic Super Nick eggs were incubated for 24 hours (h) and divided into four groups of 15 eggs each. Phe was administrated through the sub-blastoderm, and the eggs were incubated for another 24 h. All eggs were opened after 48 h of incubation, and the embryos were evaluated morphologically and histopathologically.
RESULTS: In Group 1 (control group), none exhibited neural tube defects (NTDs) (0%), 1 (6.6%) was undeveloped; in Group 2 (low dosages), 1 did not develop (6.6%); in Group 3 (normal dosages), 2 (13.4%) had NTDs, 1 (6.6%) was undeveloped; in Group 4 (high dosages), 5 (33.3%) had NTDs, 2 (13.3%) were undeveloped.
CONCLUSION: In light of the results, it was determined that the use of increasing doses of Phe led to defects in midline closure in early chicken embryos. This is the first report in the literature on Phe used in an early chicken embryo model.
Keywords : Neural tube defect, Growth retardation, Myorelaxant drug, Phenyramidol, Undeveloped embryo, Chicken embryo