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¹National Institute of Mental Health and Neurosciences (NIMHANS), Department of Neurosurgery, Bangalore, Karnataka, India DOI : 10.5137/1019-5149.JTN.22646-18.3 In side to side anastomosis and end to end (one way up) microvascular anastomosis, the back wall of the vessel is sutured first with inverted/buried knots. A common mistake made by surgeons during this step is the formation of a granny knot. In inverted suturing, cut ends of granny knot points inside the vessel lumen and interfere with the suture line. This additional Intraluminal thread may promote foreign body reaction and may act as a nidus for thrombus formation. Hence, this subtle mistake in knot formation may cause immediate and/or delayed anastomosis failure. The purpose of the study was to describe principles of inverted/buried reef knot formation. We describe in detail the methodology to form an inverted reef knot in microsurgery using paracords and a glove model. Keywords : Inverted knot, Buried knot, Reef knot, Microvascular anastomosis, Knot