Turkish Neurosurgery 2019 , Vol 29 , Num 4
How Safe is the Use of Intrathecal Fluorescein? An Experimental Study
Mahmut CAMLAR1,Caglar TURK1,Fatih OLTULU2,Merve OREN3,Aylin BUHUR2,Gurkan YIGITTURK2,Fusun OZER1,Nurperi GAZIOGLU4
1University of Health Sciences, Tepecik Training and Research Hospital, Department of Neurosurgery, Izmir, Turkey
2Ege University, Histology and Embryology Department, Bornova, Izmir, Turkey
3Erzurum Provincial Health Directorate, Public Health Services Presidency, Erzurum, Turkey
4Istanbul Bilim University, Department of Neurosurgery, Istanbul, Turkey
DOI : 10.5137/1019-5149.JTN.24085-18.3 AIM: To investigate the effects of fluorescein-sodium on neural tissues.

MATERIAL and METHODS: Twenty-one Wistar rats were randomly divided into three experimental groups: control (group 1) and fluorescein-sodium groups with different doses (groups 2 and 3). In the control group, craniectomy following with durotomy was performed with the help of a loupe microscope, and a dry sponge was overlayed to the brain tissue. In the study groups, the open dura was covered with a sponge soaked with 0.02 mg (group 2) and with 0.2 mg (group 3) fluorescein sodium following craniectomy. Three weeks postoperatively, rats were sacrificed for the histopathologic evaluations.

RESULTS: Fluorescein-induced apoptosis occurs in a dose-dependent manner in rats’ neurons. It was determined that neuron and neuroglial cell TUNEL staining was statistically different among the three groups (p<0.001). Our results indicated that fluorescein induces apoptosis, resulting in increased nuclear factor kappa beta (NF-κβ) expression in a dose-dependent manner.

CONCLUSION: Fluorescein sodium is used frequently during surgery for CSF fistulas. However, information in the literature about its safety is insufficient. Our study holds promise for the development of new studies on the reliability of this agent. Keywords : Fluorescein-sodium, Rhinorrhea, Neurotoxic effect, CSF fistula

Corresponding author : Mahmut CAMLAR, drcamlar@gmail.com