Turkish Neurosurgery 2019 , Vol 29 , Num 4
Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?
Necati KAPLAN1,Numan KARAARSLAN2,Ä°brahim YILMAZ3,Duygu YASAR SIRIN4,Feride Sinem AKGUN5,Tezcan CALISKAN2,Abdullah Talha SIMSEK2,Hanefi OZBEK3
1Istanbul Rumeli University, Corlu Reyap Hospital, Department of Neurosurgery, Tekirdag, Turkey
2Namik Kemal University School of Medicine, Department of Neurosurgery, Tekirdag, Turkey
3Istanbul Medipol University School of Medicine, Department of Medical Pharmacology, Istanbul, Turkey
4Namik Kemal University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Tekirdag, Turkey
5Istanbul Maltepe University School of Medicine, Department of Emergency Medicine, Istanbul, Turkey
DOI : 10.5137/1019-5149.JTN.24336-18.0 AIM: To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intact intervertebral disc tissue.

MATERIAL and METHODS: Cell cultures were prepared from tissues obtained from six cases who had undergone surgery due to spinal trauma. Dabigatran, an active pharmacological agent, was applied to intact annulus fibrosus (AF)/nucleus pulposus (NP) primary cell cultures from the study group. After performing cell viability, toxicity, and proliferation tests on all cultures in the control and study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), and matrix metalloproteinase (MMP)-13 and -19 expressions were measured via a real-time polymerase chain reaction (RT-PCR). Data were analyzed statistically.

RESULTS: In the proliferation assays performed on the 20th day of the study, cells in the dabigatran-supplemented group were reported to have lost 46.37% more viability than those in the control group. Expressions of all genes examined except MMP-13 were evaluated in the control group by time, but in contrast to the control group results, COMP and MMP-19 gene expressions decreased in the dabigatran-treated group. No CHAD or MMP-13 expression was noted in these cultures.

CONCLUSION: The potential for a systemically applied drug to accumulate in tissue and negatively affect surrounding tissues and microstructures must be emphasized. Keywords : Cytotoxicity, Dabigatran, Deep vein thrombosis, Intervertebral disc cells, Primary cell cultures

Corresponding author : Numan KARAARSLAN, numikara@yahoo.com