Issue release date: 12.11.2024
2China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Department of Radiology, Chongqing, China
3Children?s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Pediatrics, Department of Neurosurgery, Chongqing, China DOI : 10.5137/1019-5149.JTN.19379-16.2 AIM: To identify differentially expressed microRNAs in medulloblastoma, and to investigate their biological function.
MATERIAL and METHODS: Differentially expressed microRNAs were identified using gene chips, and significantly different microRNAs were selected for verification using real time quantitative PCR. Potential target genes and their biological pathways were predicted by bioinformatics software.
RESULTS: Our analysis identified two microRNAs, hsa-miR-208a-3p and hsa-miR-1207-5p, which were significantly downregulated in medulloblastoma. Bioinformatics analysis identified potential target genes in the Wnt and MAPK signaling pathways, including NLK, RAPGEF2, CACNA2D1, DUSP3, MAPK8IP3.
CONCLUSION: Downregulation of hsa-miR-208a-3p and hsa-miR-1207-5p may be involved in the occurrence of medulloblastoma, through modulations of the Wnt and MAPK signaling pathways.
Keywords : Medulloblastoma, hsa-miR-208a-3p, hsa-miR-1207-5p, Target gene, Wnt signaling pathway, MAPK signaling pathway