Turkish Neurosurgery 2013 , Vol 23 , Num 6
The Experimental Study of miRNA in Pituitary Adenomas
Siquan LIANG1, Li CHEN2, Huiling HUANG1, Dashi ZHI1
1Tianjin Huanhu Hospital, Department of Neurosurgery, Tianjin, China
2Tianjin Tianhe Hospital, Department of Neurology, Tianjin, China
DOI : 10.5137/1019-5149.JTN.7425-12.1 AIM: We investigated the differential miRNA expression in pituitary adenomas (both non-functioning and gonadotropin-secreting) and normal pituitaries.

MATERIAL and METHODS: RNA was extracted and purified from pituitary adenomas (10 non-functioning and 10 gonadotropin-secreting) and from two normal pituitary tissue samples. The samples were analyzed by miRNA microarray. Gene expression was measured using realtime RT-PCR with SYBR GREEN I.

RESULTS: In non-functioning pituitary adenomas, 25 miRNA genes were up-regulated (six by over 5-fold) and 15 were down-regulated (six by more than 10-fold). miR-124a was the most up-regulated gene (38.58-fold), and miR-31 the most down-regulated gene (21.5-fold). In gonadotropin-secreting pituitary adenomas, 16 miRNA genes were up-regulated (six by over 4-fold) and 13 were down-regulated (seven by more than 10-fold). miR-10b was the most up-regulated gene (48.73-fold), and miR-503 the most down-regulated gene (39.8-fold). Five genes were up-regulated in both subtypes: miR-523, miR-10b, miR-520b, miR-422a, and miR-422b. The RT-PCR results were consistent with those of the gene chips.

CONCLUSION: We established miRNA expression maps of non-functioning and gonadotropin-secreting pituitary adenomas. The most strongly differentially expressed genes were miR-124a and miR-31 in non-functioning pituitary adenomas, and miR-10b and miR-503 in gonadotropin-secreting pituitary adenomas. Keywords : Pituitary adenoma, miRNA gene, Gene chip

Corresponding author : Siquan Lıang, Liangsiquan@163.com