Turkish Neurosurgery 1994 , Vol 4 , Num 1
BLOOD-BRAIN BARRIER ALTERATIONS FOLIOWING INTRACEREBRAL GRAFTING: SOLID AND CELL SUSPENSIONS OF FETAL CENTRAL NERVOUS SYSTEM TISSUE
Nejat AKALAN1, Hakan SEKN2
1Hacettepe University School of Medicine Department of Neurosurgery, Ankara Trkiye
2Dr. Muhittin lker Adi Yardim ve Travmatoloji Hospital Clinic of Neurosurgery, Ankara Trkiye
The time course of angiogenesis and the status of the BBB within solid and cell suspension grafts of fetal CNS tissue following intracerebral transplantation is studied. Adult rats (n=40) received fetal forebrain tissue as either solid or cell suspension grafts to their corpus callosun bilaterally. The presence and the viability of the grafts was examined in fifty micron corenal sections treated by cresyl violet (Nissl) and acetylcholinesterase (AChE) histochemistry. BBB permeability within the grafts were determined by horseradish perexide (HRP) leakege given systemically on the first. Third, seventh and tenth post-operative days, to four separate groups of rats (n:10). HRP leakage was detected in both solid and cell suspension grafts on the first and third days following transplantation indicating a distrupted BBB. By the seventh postoperative day, no HRP reaction was detected. These results suggest that the BBB permeability of the vessels within intracerebral solid grafts depends on the transplanted tissue itself while the host CNS tissue prevokes the formation of an intact BBB for cell suspension grafts. Keywords : Angiogenesis, Blood-brain barrier, Horseradish peroxide, Neural transplantion
Corresponding author : Nejat Akalan