Turkish Neurosurgery 2019 , Vol 29 , Num 1
Does the Anti-Migraine Drug Rizatriptan Affect Early Neural Tube Development in Chick Embryos?
Murat SAYIN1,Gulsen Seren GURGEN2,Sevgi Sdka SAYIN3,Gonul GUVENC1,Nurullah YUCEER1
1Izmir Katip Celebi University, School of Medicine, Department of Neurosurgery, Izmir, Turkey
2Celal Bayar University, School of Medicine, Department of Histology and Embryology, Manisa, Turkey
3Izmir Bozyaka Education and Research Hospital, Neurology Clinic, Izmir, Turkey
DOI : 10.5137/1019-5149.JTN.23431-18.1 AIM: To investigate the impact of rizatriptan on neural tube development using early chick embryos as a model organism.

MATERIAL and METHODS: A total of 36 pathogen-free Leghorn chicken eggs were selected and categorized in three groups: sham, therapeutic, and supra-therapeutic. After 24 hours, the eggs were opened and injected with sterile drugs, and then reclosed using plastic tape. After a period of 72 hours, the eggs were opened and assessed using the Hamburger?Hamilton chick embryology classification method. TUNEL staining was used to identify apoptosis, and hematoxylin?eosin staining was used to investigate neural tube closure.

RESULTS: Treatment with rizatriptan significantly slowed down neural tube development. The supra-therapeutic group showed neural tube closure defects.

CONCLUSION: Rizatriptan had a negative effect on neural tube closure. Further research is needed to identify a safe and effective drug for treating migraines during pregnancy. Keywords : Chick embryo, Migraine, Neural tube defect, Pregnancy, Rizatriptan

Corresponding author : Murat SAYIN, smsayin@gmail.com